| Q. | "What would you do to make a standard curve so you can convert absorbance units into cell density?"? | Related Search:
Higher Education (University ) | | | microbiology
| | A. | Generic is a from of Dual-Homed. in most cases Logic-Subsystem that make convent absorbance units into cells. most of the time u have to Inherent, Checksum into the density to make the system complete. i hope that help u out. | | | |
| Q. | I need to convert the following rates from AU min-1 (absorbance units) to mole l-1 min-1...? | Related Search:
Chemistry | | | I performed reactions to see the rate PNP is formed. i need to convert the following rates from absorbtion units given that the molar extinction coefficient for PNP at 410 nm is 18000 M-1 cm-1.
0.002
0.010
0.020
0.063
0.098
0.123
I know the beer-lamberts law is used somewhere, im just not sure where.
Cheers in advance.
| | A. | Beer Lambert Law is A = Ecl where A is absirbance, E is molar extinction coefficient, c inconcentration and L is cell pathlength
So c = A/El
Assuming your cell pathlength was the standard 1 cm, then all you have to do is divide your absorbance values by E to get concentration in mol L-1. | | | |
| Q. | what are the units for absorbance? | Related Search:
Medicine | | | im talking about electromagnetic absorbance
| | A. | light, for example is electromagnetic. The absorbance term for light, although dimensionless is defined as -log(T) where T is the % transmittance through the sample. | | | |
| Q. | How dod you calculate the concentration of a protein from the absorbance? | Related Search:
Biology | | | The Absorbance was 0.687
The molar extinction coefficient is 6220
the protein was diluted 100-fold
I understand that, using beer's law, c = A x k, the concentration is 3775.5 (not sure about units)
I'm not sure what to multiply by to get the right correction factor.
Thank you
| | A. | From what I remember Beer's law says
absorbance = molar extinction x length traveled x concentration
thus to find the concentration you would need to divide the absorbance by the molar extinction coefficient and the length the light traveled in your measurement (the length of the cuvette you used)
Multiply this concentration with the dilution factor and you'll have the initial concentration.
The unit's mol/L btw
have fun | | | |
| Q. | Why is absorbance a more widely used unit than transmittance? | Related Search:
Chemistry | | | please explain how n why u got your answer
| | A. | Absorbance is linearly related to concentration while transmittance is not. Beer-Lambert law shows the linear relationship between concentration and absorbance. | | | |
| Q. | Unit for absorbance from spectrometer experiments? | Related Search:
Chemistry | | | What is the unit of the absorbance readings shown by the spectrometer?
| | A. | There are no units for absorbance in spectrometer readings as the intensities that are displayed are just numbers. The reason for this is that Absorption is defined as a ratio between the amount of light going in versus coming out after being through the sample. In short, absorbance is unitless. | | | |
| Q. | Chemistry lab -- Does changing width or height of cuvette change the amount of absorbance? | Related Search:
Chemistry | | | We have a unit that collects the absorbance of samples in a cuvette and displays it on a laptop connected via USB:
Would changing the width of the cuvette alter the absorbance? If so, how?
Would changing the height of the cuvette alter the absorbance? If so, how?
| | A. | The amount of light absorbed is proportional to the distance the light travels through the solution. | | | |
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